The intranuclear binding of testosterone and 5α-androstan-17β-ol-3-one by rat prostate
N Bruchovsky, JD Wilson - Journal of Biological Chemistry, 1968 - Elsevier
N Bruchovsky, JD Wilson
Journal of Biological Chemistry, 1968•ElsevierThe intranuclear binding of radioactive hormone in prostate after the intravenous
administration of testosterone-1, 2-3 H to rats has been studied. Nuclei obtained by sucrose
density gradient centrifugation were extracted with buffer containing 0.6 m NaCl, and the
soluble radioactivity was separated into bound and free fractions by gel filtration on
Sephadex G-25 or G-200. As early as 15 min after testosterone administration, the
radioactivity recovered in the bound form was predominantly dihydrotestosterone. By gel …
administration of testosterone-1, 2-3 H to rats has been studied. Nuclei obtained by sucrose
density gradient centrifugation were extracted with buffer containing 0.6 m NaCl, and the
soluble radioactivity was separated into bound and free fractions by gel filtration on
Sephadex G-25 or G-200. As early as 15 min after testosterone administration, the
radioactivity recovered in the bound form was predominantly dihydrotestosterone. By gel …
The intranuclear binding of radioactive hormone in prostate after the intravenous administration of testosterone-1,2-3H to rats has been studied. Nuclei obtained by sucrose density gradient centrifugation were extracted with buffer containing 0.6 m NaCl, and the soluble radioactivity was separated into bound and free fractions by gel filtration on Sephadex G-25 or G-200. As early as 15 min after testosterone administration, the radioactivity recovered in the bound form was predominantly dihydrotestosterone.
By gel filtration of nuclear extracts on Sephadex G-200 and by the use of digestive enzymes, it was shown that dihydrotestosterone was bound to an acidic nuclear protein. Finally, several characteristics of this binding phenomenon were defined; the binding was stable to freezing for as long as 8 days, stable to short term incubation at 20° but not at 37°, and partially stable to repeated gel filtration on Sephadex.
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