A low-viscosity epoxy resin embedding medium for electron microscopy

AR Spurr - Journal of ultrastructure research, 1969 - Elsevier
A low-viscosity embedding medium based on ERL-4206 is recommended for use in electron
microscopy. The composition is: ERL-4206 (vinyl cyclohexene dioxide) 10 g, DER 736
(diglycidyl ether of polypropylene glycol) 6 g, NSA (nonenyl succinic anhydride) 26 g, and S-
1 (dimethylaminoethanol or DMAE) 0.4 g. The medium is easily and rapidly prepared by
dispensing the components, in turn by weight, into a single flask. The relatively low viscosity
of the medium (60 cP) permits rapid mixing by shaking and swirling. The medium is …