Rapid generation of amyloid from native proteins in vitro

SM Dorta-Estremera, J Li, W Cao - JoVE (Journal of Visualized …, 2013 - jove.com
Proteins carry out crucial tasks in organisms by exerting functions elicited from their specific
three dimensional folds. Although the native structures of polypeptides fulfill many purposes,
it is now recognized that most proteins can adopt an alternative assembly of beta-sheet rich
amyloid. Insoluble amyloid fibrils are initially associated with multiple human ailments, but
they are increasingly shown as functional players participating in various important cellular
processes. In addition, amyloid deposited in patient tissues contains nonproteinaceous …