BAFF enhances chemotaxis of primary human B cells: a particular synergy between BAFF and CXCL13 on memory B cells

G Badr, G Borhis, EA Lefevre, N Chaoul… - Blood, The Journal …, 2008 - ashpublications.org
G Badr, G Borhis, EA Lefevre, N Chaoul, F Deshayes, V Dessirier, G Lapree, A Tsapis…
Blood, The Journal of the American Society of Hematology, 2008ashpublications.org
B-cell–activating factor of the TNF family,(BAFF), and a proliferation-inducing ligand (APRIL)
regulate B-lymphocyte survival and activation. We report that BAFF, but not APRIL,
increased the chemotactic response of primary human B cells to CCL21, CXCL12, and
CXCL13. The BAFF-induced increase in B-cell chemotaxis was totally abolished by
blockade of BAFF-R and was strongly dependent on the activation of PI3K/AKT, NF-κB, and
p38MAPK pathways. BAFF had similar effects on the chemotaxis of naive and memory B …
B-cell–activating factor of the TNF family, (BAFF), and a proliferation-inducing ligand (APRIL) regulate B-lymphocyte survival and activation. We report that BAFF, but not APRIL, increased the chemotactic response of primary human B cells to CCL21, CXCL12, and CXCL13. The BAFF-induced increase in B-cell chemotaxis was totally abolished by blockade of BAFF-R and was strongly dependent on the activation of PI3K/AKT, NF-κB, and p38MAPK pathways. BAFF had similar effects on the chemotaxis of naive and memory B cells in response to CCL21 but increased more strongly that of memory B cells to CXCL13 than that of naive B cells. Our findings indicate a previously unreported role for the BAFF/BAFF-R pair in mature B-cell chemotaxis. The synergy between CXCL13 and BAFF produced by stromal cells and follicular dendritic cells may have important implications for B-cell homeostasis, the development of normal B-cell areas, and for the formation of germinal center–like follicles that may be observed in various autoimmune diseases.
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