Traditionally, the identification and quantification of eosinophils in inflammatory tissues and
exudates has been primarily based upon morphologic criteria and manual counting. In this
study, we describe a new flow cytometry-based assay to enumerate eosinophils present in
murine bronchoalveolar lavage fluid (BAL) and lung parenchyma obtained from the
normal/non-inflamed respiratory tract, following experimentally-induced allergic pulmonary
inflammation, and during experimental infection with respiratory syncytial virus (RSV). By …