[HTML][HTML] DNA binding of repressor nuclear factor-κB p50/p50 depends on phosphorylation of Ser337 by the protein kinase A catalytic subunit
H Guan, S Hou, RP Ricciardi - Journal of Biological Chemistry, 2005 - ASBMB
The NF-κB p50/p50 homodimer is mainly associated with transcriptional repression.
Previously, we demonstrated that phosphorylation of NF-κB p50 Ser 337 is critical for DNA
binding. Here, we report that p50 Ser 337 is constitutively phosphorylated by the protein
kinase A catalytic subunit (PKAc) in three different cell types, which may account for the
constant binding of p50/p50 to DNA in unstimulated cells. This was demonstrated first by
showing that treatment of cells with PKAc-specific inhibitors blocked p50/p50 DNA binding …
Previously, we demonstrated that phosphorylation of NF-κB p50 Ser 337 is critical for DNA
binding. Here, we report that p50 Ser 337 is constitutively phosphorylated by the protein
kinase A catalytic subunit (PKAc) in three different cell types, which may account for the
constant binding of p50/p50 to DNA in unstimulated cells. This was demonstrated first by
showing that treatment of cells with PKAc-specific inhibitors blocked p50/p50 DNA binding …