Binucleation and polyploidization patterns in developmental and regenerative rat liver growth
P Gerlyng, A Åbyholm, T Grotmol, B Erikstein… - Cell …, 1993 - Wiley Online Library
Cell proliferation, 1993•Wiley Online Library
The hepatocellular binucleation rate, measured as the percentage of binuclear cells
amongst newly formed bromodeoxyuridine‐labelled and immunostained collage‐nase‐
isolated rat hepatocytes, decreased from 12% to 4% between days 30 and 40 after birth,
rose to 20% between days 50 and 60, and then declined again to the adult rate of about
10% at day 80. During regenerative growth following a two‐thirds partial hepatectomy, the
rate of binucleation declined to about 3%, causing the fraction of binuclear cells to fall from …
amongst newly formed bromodeoxyuridine‐labelled and immunostained collage‐nase‐
isolated rat hepatocytes, decreased from 12% to 4% between days 30 and 40 after birth,
rose to 20% between days 50 and 60, and then declined again to the adult rate of about
10% at day 80. During regenerative growth following a two‐thirds partial hepatectomy, the
rate of binucleation declined to about 3%, causing the fraction of binuclear cells to fall from …
Abstract
The hepatocellular binucleation rate, measured as the percentage of binuclear cells amongst newly formed bromodeoxyuridine‐labelled and immunostained collage‐nase‐isolated rat hepatocytes, decreased from 12% to 4% between days 30 and 40 after birth, rose to 20% between days 50 and 60, and then declined again to the adult rate of about 10% at day 80. During regenerative growth following a two‐thirds partial hepatectomy, the rate of binucleation declined to about 3%, causing the fraction of binuclear cells to fall from 27% (before hepactectomy) to 5% (at 45 h after hepactectomy) as pre‐existing binuclear cells replicated and formed mononuclear daughter cells. Essentially all (97%) hepatocytes replicated at least once, starting their DNA synthesis at around 13 h and reaching a peak at 30 h, irrespective of ploidy and nuclearity. At later time points, the diploid hepatocytes had a higher labelling index than the polyploid cells, suggesting a greater tendency to go through several cell cycles.
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