RNA targets of wild-type and mutant FET family proteins
Nature structural & molecular biology, 2011•nature.com
Abstract FUS, EWSR1 and TAF15, constituting the FET protein family, are abundant, highly
conserved RNA-binding proteins with important roles in oncogenesis and neuronal disease,
yet their RNA targets and recognition elements are unknown. Using PAR-CLIP, we defined
global RNA targets for all human FET proteins and two ALS-causing human FUS mutants.
FET members showed similar binding profiles, whereas FUS mutants showed a drastically
altered binding pattern, consistent with changes in subcellular localization.
conserved RNA-binding proteins with important roles in oncogenesis and neuronal disease,
yet their RNA targets and recognition elements are unknown. Using PAR-CLIP, we defined
global RNA targets for all human FET proteins and two ALS-causing human FUS mutants.
FET members showed similar binding profiles, whereas FUS mutants showed a drastically
altered binding pattern, consistent with changes in subcellular localization.
Abstract
FUS, EWSR1 and TAF15, constituting the FET protein family, are abundant, highly conserved RNA-binding proteins with important roles in oncogenesis and neuronal disease, yet their RNA targets and recognition elements are unknown. Using PAR-CLIP, we defined global RNA targets for all human FET proteins and two ALS-causing human FUS mutants. FET members showed similar binding profiles, whereas FUS mutants showed a drastically altered binding pattern, consistent with changes in subcellular localization.
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