Experimental tests to definitively determine ubiquitylation of a substrate
J Bloom, M Pagano - Methods in enzymology, 2005 - Elsevier
Ubiquitin‐mediated proteolysis is a major pathway of protein degradation that regulates
numerous cellular processes. An understanding of the circumstances that contribute to the
ubiquitylation of a specific protein can yield vast insight into its regulation. This article
examines multiple procedures that explain whether a protein is ubiquitylated and suggests
methods to investigate the factors that specifically target the substrate for ubiquitylation, as
well as the site of ubiquitin conjugation.
numerous cellular processes. An understanding of the circumstances that contribute to the
ubiquitylation of a specific protein can yield vast insight into its regulation. This article
examines multiple procedures that explain whether a protein is ubiquitylated and suggests
methods to investigate the factors that specifically target the substrate for ubiquitylation, as
well as the site of ubiquitin conjugation.