[HTML][HTML] Inhibition of Akt kinase by cell-permeable ceramide and its implications for ceramide-induced apoptosis
Ceramide is an important lipid messenger involved in mediating a variety of cell functions
including apoptosis. However, mechanisms responsible for ceramide-induced apoptosis
remain unclear. We investigated the possibility that ceramide may decrease antiapoptotic
signaling in cells by inhibiting Akt kinase activity. Our data show that C 2-ceramide induces
apoptosis in HMN1 motor neuron cells and decreases both basal and insulin-or serum-
stimulated Akt kinase activity 65–70%. These results are consistent with decreased Akt …
including apoptosis. However, mechanisms responsible for ceramide-induced apoptosis
remain unclear. We investigated the possibility that ceramide may decrease antiapoptotic
signaling in cells by inhibiting Akt kinase activity. Our data show that C 2-ceramide induces
apoptosis in HMN1 motor neuron cells and decreases both basal and insulin-or serum-
stimulated Akt kinase activity 65–70%. These results are consistent with decreased Akt …