[HTML][HTML] Direct effect of ceramide on the mitochondrial electron transport chain leads to generation of reactive oxygen species: role of mitochondrial glutathione
Ceramide is a sphingolipid that is generated in the signaling of inflammatory cytokines such
as tumor necrosis factor (TNF), which exerts many functional roles depending on the cell
type where it is produced. Since TNF cytotoxicity is mediated by overproduction of reactive
oxygen species from mitochondria, we have examined the role of ceramide in generation of
oxidative stress in isolated rat liver mitochondria. The present studies demonstrate that
addition of N-acetylsphingosine (C 2-ceramide) to mitochondria led to an increase of …
as tumor necrosis factor (TNF), which exerts many functional roles depending on the cell
type where it is produced. Since TNF cytotoxicity is mediated by overproduction of reactive
oxygen species from mitochondria, we have examined the role of ceramide in generation of
oxidative stress in isolated rat liver mitochondria. The present studies demonstrate that
addition of N-acetylsphingosine (C 2-ceramide) to mitochondria led to an increase of …