Roles of active oxygen species in glomerular epithelial cell injury in vitro caused by puromycin aminonucleoside
M Kawaguchi, M Yamada, H Wada, T Okigaki - Toxicology, 1992 - Elsevier
The mechanism of puromycin aminonucleoside (PAN)-induced nephrosis has not yet been
well defined. In the present study, we examined the protective effect of active oxygen
scavengers on the PAN-induced injury of cultured rat glomerular epithelial cells (GECs) and
the generation of active oxygen species in PAN-treated GFCs. When exposed to PAN (>-25
μ/ml), cellular damage occurred in a time-and dose-dependent manner as evaluated by both
the LDH release and MTT colorimetric assays. Concomitant addition of either the hydrogen …
well defined. In the present study, we examined the protective effect of active oxygen
scavengers on the PAN-induced injury of cultured rat glomerular epithelial cells (GECs) and
the generation of active oxygen species in PAN-treated GFCs. When exposed to PAN (>-25
μ/ml), cellular damage occurred in a time-and dose-dependent manner as evaluated by both
the LDH release and MTT colorimetric assays. Concomitant addition of either the hydrogen …