Corneal endothelial cell matrix promotes expression of differentiated features of retinal pigmented epithelial cells: implication of laminin and basic fibroblast growth …
PA Campochiaro, SF Hackett - Experimental eye research, 1993 - Elsevier
Human retinal pigmented epithelial (RPE) cells cultured on plastic, unlike RPE in situ, often
fail to density arrest, do not produce melanin, and do not express mRNA for cellular
retinaldehyde binding protein (CRALBP). When human RPE are cultured on the
extracellular matrix produced by bovine corneal endothelial cells for 1 week or human RPE
for 3 weeks, they density arrest, assume a differentiated morphology, and produce pigment
and mRNA for CRALBP (all differentiated features of RPE). Human RPE grown on a laminin …
fail to density arrest, do not produce melanin, and do not express mRNA for cellular
retinaldehyde binding protein (CRALBP). When human RPE are cultured on the
extracellular matrix produced by bovine corneal endothelial cells for 1 week or human RPE
for 3 weeks, they density arrest, assume a differentiated morphology, and produce pigment
and mRNA for CRALBP (all differentiated features of RPE). Human RPE grown on a laminin …