[CITATION][C] [7] Preparation of isolated fat cells and fat cell “ghost”; methods for assaying adenylate cyclase activity and levels of cyclic AMP
M Rodbell, G Krishna - Methods in enzymology, 1974 - Elsevier
A variety of peptide hormones and biogenic amines stimulate the activity of adenylate
cyclase, a membrane-bound regulatory enzyme system that catalyzes the production of
cyclic AMP from ATP. 1 The role of cyclic AMP in the regulation of cellular metabolism has
been reviewed extensivelyY Two approaches are generally employed to investigate the
activity and response of adenylate cyclase to hormones. One approach is to measure the
levels of cyclic AMP generated in intact cells or tissues. This approach provides information …
cyclase, a membrane-bound regulatory enzyme system that catalyzes the production of
cyclic AMP from ATP. 1 The role of cyclic AMP in the regulation of cellular metabolism has
been reviewed extensivelyY Two approaches are generally employed to investigate the
activity and response of adenylate cyclase to hormones. One approach is to measure the
levels of cyclic AMP generated in intact cells or tissues. This approach provides information …