Evaluation of the substrate specificity of human mast cell tryptase βI and demonstration of its importance in bacterial infections of the lung
C Huang, GT De Sanctis, PJ O'Brien, JP Mizgerd… - Journal of Biological …, 2001 - ASBMB
Human pulmonary mast cells (MCs) express tryptases α and βI, and both granule serine
proteases are exocytosed during inflammatory events. Recombinant forms of these tryptases
were generated for the first time to evaluate their substrate specificities at the biochemical
level and then to address their physiologic roles in pulmonary inflammation. Analysis of a
tryptase-specific, phage display peptide library revealed that tryptase βI prefers to cleave
peptides with 1 or more Pro residues flanked by 2 positively charged residues. Although …
proteases are exocytosed during inflammatory events. Recombinant forms of these tryptases
were generated for the first time to evaluate their substrate specificities at the biochemical
level and then to address their physiologic roles in pulmonary inflammation. Analysis of a
tryptase-specific, phage display peptide library revealed that tryptase βI prefers to cleave
peptides with 1 or more Pro residues flanked by 2 positively charged residues. Although …
