Streamlined procedure for the production of normal and altered versions of recombinant human proinsulin
RB Mackin - Protein expression and purification, 1999 - Elsevier
A method for the simplified, reproducible production of both normal and altered versions of
human proinsulin has been developed. A polyhistidine/proinsulin fusion protein was
expressed using a prokaryotic expression system and partially purified by affinity
chromatography. Disulfide bonds within the polypeptide were formed prior to removal of the
affinity tag. The proinsulin cleaved from the fusion protein was then subjected to a final
purification step of semipreparative reversed-phase high-performance liquid …
human proinsulin has been developed. A polyhistidine/proinsulin fusion protein was
expressed using a prokaryotic expression system and partially purified by affinity
chromatography. Disulfide bonds within the polypeptide were formed prior to removal of the
affinity tag. The proinsulin cleaved from the fusion protein was then subjected to a final
purification step of semipreparative reversed-phase high-performance liquid …
