[HTML][HTML] Down-regulation of Rac activity during β2 integrin-mediated adhesion of human neutrophils
K Dib, F Melander, L Axelsson, MC Dagher… - Journal of Biological …, 2003 - ASBMB
In human neutrophils, β 2 integrin engagement mediated a decrease in GTP-bound Rac1
and Rac2. Pretreatment of neutrophils with LY294002 or PP1 (inhibiting phosphatidylinositol
3-kinase (PI 3-kinase) and Src kinases, respectively) partly reversed the β 2 integrin-induced
down-regulation of Rac activities. In contrast, β 2 integrins induced stimulation of Cdc42 that
was independent of Src family members. The PI 3-kinase dependence of the β 2 integrin-
mediated decrease in GTP-bound Rac could be explained by an enhanced Rac-GAP …
and Rac2. Pretreatment of neutrophils with LY294002 or PP1 (inhibiting phosphatidylinositol
3-kinase (PI 3-kinase) and Src kinases, respectively) partly reversed the β 2 integrin-induced
down-regulation of Rac activities. In contrast, β 2 integrins induced stimulation of Cdc42 that
was independent of Src family members. The PI 3-kinase dependence of the β 2 integrin-
mediated decrease in GTP-bound Rac could be explained by an enhanced Rac-GAP …
