Rac activation induces NADPH oxidase activity in transgenic COSphox cells, and the level of superoxide production is exchange factor-dependent
MO Price, SJ Atkinson, UG Knaus… - Journal of Biological …, 2002 - ASBMB
Transient expression of constitutively active Rac1 derivatives,(G12V) or (Q61L), was
sufficient to induce phagocyte NADPH oxidase activity in a COS-7 cell model in which
human cDNAs for essential oxidase components, gp91 phox, p22 phox, p47 phox, and p67
phox, were expressed as stable transgenes. Expression of constitutively active Rac1 in"
COS phox" cells induced translocation of p47 phox and p67 phox to the membrane.
Furthermore, translocation of p47 phox was induced in the absence of p67 phox expression …
sufficient to induce phagocyte NADPH oxidase activity in a COS-7 cell model in which
human cDNAs for essential oxidase components, gp91 phox, p22 phox, p47 phox, and p67
phox, were expressed as stable transgenes. Expression of constitutively active Rac1 in"
COS phox" cells induced translocation of p47 phox and p67 phox to the membrane.
Furthermore, translocation of p47 phox was induced in the absence of p67 phox expression …
