[HTML][HTML] Cellular response to an antisense-mediated shift of Bcl-x pre-mRNA splicing and antineoplastic agents
DR Mercatante, JL Mohler, R Kole - Journal of Biological Chemistry, 2002 - ASBMB
Overexpression of Bcl-xL, an anti-apoptotic member of the Bcl-2 family, negatively correlates
with the sensitivity of various cancers to chemotherapeutic agents. We show here that high
levels of expression of Bcl-xL promoted apoptosis of cells treated with an antisense
oligonucleotide (5′ Bcl-x AS) that shifts the splicing pattern of Bcl-x pre-mRNA from the anti-
apoptotic variant, Bcl-xL, to the pro-apoptotic variant, Bcl-xS. This surprising finding
illustrates the advantage of antisense-induced modulation of alternative splicing versus …
with the sensitivity of various cancers to chemotherapeutic agents. We show here that high
levels of expression of Bcl-xL promoted apoptosis of cells treated with an antisense
oligonucleotide (5′ Bcl-x AS) that shifts the splicing pattern of Bcl-x pre-mRNA from the anti-
apoptotic variant, Bcl-xL, to the pro-apoptotic variant, Bcl-xS. This surprising finding
illustrates the advantage of antisense-induced modulation of alternative splicing versus …
