Trans splicing of mRNA precursors
D Solnick - Cell, 1985 - cell.com
D Solnick
Cell, 1985•cell.comUsing modified adenovirus and β-globin transcription units, I constructed pairs of transcripts
that contained mutually complementary sequences in their introns. Upon incubation of the
annealed transcripts in a cell-free splicing system, an exon from one member of the dimeric
complex was accurately spliced to an exon from the other. The occurrence of such
intermolecular, or trans, splicing in vitro raises the possibility that some of the mRNAs in a
cell acquire exons from more than one primary transcript.
that contained mutually complementary sequences in their introns. Upon incubation of the
annealed transcripts in a cell-free splicing system, an exon from one member of the dimeric
complex was accurately spliced to an exon from the other. The occurrence of such
intermolecular, or trans, splicing in vitro raises the possibility that some of the mRNAs in a
cell acquire exons from more than one primary transcript.
Summary
Using modified adenovirus and β-globin transcription units, I constructed pairs of transcripts that contained mutually complementary sequences in their introns. Upon incubation of the annealed transcripts in a cell-free splicing system, an exon from one member of the dimeric complex was accurately spliced to an exon from the other. The occurrence of such intermolecular, or trans, splicing in vitro raises the possibility that some of the mRNAs in a cell acquire exons from more than one primary transcript.
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