[HTML][HTML] Interferon-γ-dependent nuclear import of Stat1 is mediated by the GTPase activity of Ran/TC4
T Sekimoto, K Nakajima, T Tachibana, T Hirano… - Journal of Biological …, 1996 - ASBMB
In response to interferon-γ (IFN-γ), Stat1 enters the nucleus, where it activates transcription.
In order to better understand the mechanism of the extracellular signal-induced protein
import into the nucleus, we have established an in vivo assay system that uses recombinant
Stat1 protein as a model transport substrate. Using this system, we found that Stat1 is
actively transported through the nuclear pores in an IFN-γ-dependent manner and tyrosine
(Tyr 701) phosphorylation of Stat1 is actually required for its nuclear import. When the …
In order to better understand the mechanism of the extracellular signal-induced protein
import into the nucleus, we have established an in vivo assay system that uses recombinant
Stat1 protein as a model transport substrate. Using this system, we found that Stat1 is
actively transported through the nuclear pores in an IFN-γ-dependent manner and tyrosine
(Tyr 701) phosphorylation of Stat1 is actually required for its nuclear import. When the …