Nicotinic acetylcholine receptors (AChRs) from muscle bind α‐bungarotoxin (αBgt) and are composed of four kinds of subunits, whereas AChRs from mammalian brains do not bind αBgt and are composed of two kinds of subunits. αBgt‐binding proteins whose function is unknown are also found in brain. All these proteins belong to the same gene family. The human medulloblastoma cell line TE671 expresses a functional AChR which binds αBgt. Surprisingly, the AChR of this neuron‐derived cell line has electrophysiological, immunological and biochemical properties different from neuronal AChRs and very similar to muscle AChRs. The TE671 AChR binds αBgt, but is different from αBgt‐binding proteins in brain. Here we show that TE671 expresses the α‐subunit mRNA coding for the muscle AChR, thereby proving that TE671 expresses a muscle‐type AChR that is not expressed in adult brain. The isolated cDNA clones should prove useful for expression of large amounts of human muscle‐type AChR α‐subunit protein for studies of the autoimmune response to muscle AChRs in human myasthenia gravis.