Expression and purification of recombinant human SPARC produced by baculovirus
AD Bradshaw, JA Bassuk, A Francki… - Molecular Cell Biology …, 2000 - Elsevier
SPARC (secreted protein acidic and rich in cysteine/osteonectin/BM-40), a matrix-
associated protein, disrupts cell adhesion and inhibits the proliferation of many cultured
cells. We report the expression of recombinant human protein (rhSPARC) in a baculovirus
expression system. This procedure routinely yields∼ 1 mg of purified protein per 500 ml of
culture supernate. rhSPARC produced by insect cells migrates at the appropriate molecular
weight under reducing and nonreducing conditions. The rhSPARC purified from insect cell …
associated protein, disrupts cell adhesion and inhibits the proliferation of many cultured
cells. We report the expression of recombinant human protein (rhSPARC) in a baculovirus
expression system. This procedure routinely yields∼ 1 mg of purified protein per 500 ml of
culture supernate. rhSPARC produced by insect cells migrates at the appropriate molecular
weight under reducing and nonreducing conditions. The rhSPARC purified from insect cell …
