Mutant and wild type human α-synucleins assemble into elongated filaments with distinct morphologies in vitro
BI Giasson, K Uryu, JQ Trojanowski… - Journal of Biological …, 1999 - ASBMB
α-Synuclein is a soluble presynaptic protein which is pathologically redistributed within
intracellular lesions characteristic of several neurodegenerative diseases. Here we
demonstrate that wild type and two mutant forms of α-synuclein linked to familial Parkinson's
disease (Ala 30→ Pro and Ala 53→ Thr) self-aggregate and assemble into 10–19-nm-wide
filaments with distinct morphologies under definedin vitro conditions. Immunogold labeling
demonstrates that the central region of all these filaments are more robustly labeled than the …
intracellular lesions characteristic of several neurodegenerative diseases. Here we
demonstrate that wild type and two mutant forms of α-synuclein linked to familial Parkinson's
disease (Ala 30→ Pro and Ala 53→ Thr) self-aggregate and assemble into 10–19-nm-wide
filaments with distinct morphologies under definedin vitro conditions. Immunogold labeling
demonstrates that the central region of all these filaments are more robustly labeled than the …
