UVB irradiation up-regulates serine palmitoyltransferase in cultured human keratinocytes
The enzyme serine palmitoyltransferase (SPT; EC 2.3. 1.50), which catalyzes the first
committed and rate-limiting step in sphingolipid synthesis, is up-regulated in the epidermis
as part of the homeostatic repair in response to permeability barrier perturbation. Moreover,
UVB exposure, which also perturbs the barrier, up-regulates sphingolipid synthesis, but the
basis for this increase is not known. The recent isolation of cDNAs for SPT (ie, LCB1 and
LCB2) allow molecular regulation studies to be performed. Therefore, we determined …
committed and rate-limiting step in sphingolipid synthesis, is up-regulated in the epidermis
as part of the homeostatic repair in response to permeability barrier perturbation. Moreover,
UVB exposure, which also perturbs the barrier, up-regulates sphingolipid synthesis, but the
basis for this increase is not known. The recent isolation of cDNAs for SPT (ie, LCB1 and
LCB2) allow molecular regulation studies to be performed. Therefore, we determined …
