Synthetic Copolymer I and Myelin Basic Protein Do Not Require Processing Prior to Binding to Class II Major Histocompatibility Complex Molecules on Living Antigen …
M Fridkis-Hareli, D Teitelbaum, R Arnon, M Sela - Cellular immunology, 1995 - Elsevier
In the present study we attempted to examine whether copolymer 1 (Cop 1), a synthetic
basic random copolymer of amino acids (a candidate drug for multiple sclerosis (MS)), and
myelin basic protein (MBP) undergo processing prior to their binding to MHC class II
molecules on antigen-presenting cells (APC). The direct binding of biotinylated Cop 1 and
MBP to living APC was monitored by flow cytometry using phycoerythrin (PE)-streptavidin.
The time course for either Cop 1 or MBP binding was similar at 37° C and on ice. Both Cop 1 …
basic random copolymer of amino acids (a candidate drug for multiple sclerosis (MS)), and
myelin basic protein (MBP) undergo processing prior to their binding to MHC class II
molecules on antigen-presenting cells (APC). The direct binding of biotinylated Cop 1 and
MBP to living APC was monitored by flow cytometry using phycoerythrin (PE)-streptavidin.
The time course for either Cop 1 or MBP binding was similar at 37° C and on ice. Both Cop 1 …