On the uptake of biotin by the rat renal tubule
PD Spencer, KS Roth - Biochemical medicine and metabolic biology, 1988 - Elsevier
Little is known of biotin handling by transporting epithelium. Accordingly, we have examined
the characteristics of biotin uptake by rat renal tubular epithelium. Renal cortical slices
showed concentrative, temperature-sensitive uptake of biotin. Renal brushborder membrane
vesicles exhibited an “overshoot” phenomenon with uptake of 1.9 nm biotin in the presence
of a 100 mm NaCl gradient. This overshoot was reduced in magnitude with reduction of the
sodium gradient to 50 m m. Biocytin significantly reduced uptake by the vesicles …
the characteristics of biotin uptake by rat renal tubular epithelium. Renal cortical slices
showed concentrative, temperature-sensitive uptake of biotin. Renal brushborder membrane
vesicles exhibited an “overshoot” phenomenon with uptake of 1.9 nm biotin in the presence
of a 100 mm NaCl gradient. This overshoot was reduced in magnitude with reduction of the
sodium gradient to 50 m m. Biocytin significantly reduced uptake by the vesicles …
