[HTML][HTML] Advanced analysis of biotin metabolites in body fluids allows a more accurate measurement of biotin bioavailability and metabolism in humans
J Zempleni, DM Mock - The Journal of nutrition, 1999 - Elsevier
In previous studies, the bioavailability of biotin in humans was estimated from the recovery of
biotin in urine; urinary biotin was measured by microbial growth assays or assays of avidin-
binding activity. These assays underestimate concentrations of biotin metabolites, which
originate from β-oxidation, sulfur oxidation or a combination. We have developed an
HPLC/avidin-binding assay that is specific for biotin and its metabolites. With the use of the
HPLC/avidin-binding assay, TLC and derivatization with p-dimethylaminocinnamaldehyde …
biotin in urine; urinary biotin was measured by microbial growth assays or assays of avidin-
binding activity. These assays underestimate concentrations of biotin metabolites, which
originate from β-oxidation, sulfur oxidation or a combination. We have developed an
HPLC/avidin-binding assay that is specific for biotin and its metabolites. With the use of the
HPLC/avidin-binding assay, TLC and derivatization with p-dimethylaminocinnamaldehyde …
