Isolation and culturing of hepatocytes from human livers
AP Li, MA Roque, DJ Beck, DL Kaminski - Journal of tissue culture …, 1992 - Springer
AP Li, MA Roque, DJ Beck, DL Kaminski
Journal of tissue culture methods, 1992•SpringerUsing a modified collagenase digestion procedure, we were successful in the isolation of
viable hepatocytes from human liver surgical wastes, unused transplantable livers, and
diseased livers from transplant recipients. The modified procedures for isolation involved
collagenase perfusion of a sample of limited size (< 50 g) with only one cut surface,
perfusion via only one blood vessel, the use of an appropriate amount of collagenase, as
well as manual “massaging” of the liver sample during critical stages of the collagenase …
viable hepatocytes from human liver surgical wastes, unused transplantable livers, and
diseased livers from transplant recipients. The modified procedures for isolation involved
collagenase perfusion of a sample of limited size (< 50 g) with only one cut surface,
perfusion via only one blood vessel, the use of an appropriate amount of collagenase, as
well as manual “massaging” of the liver sample during critical stages of the collagenase …
Summary
Using a modified collagenase digestion procedure, we were successful in the isolation of viable hepatocytes from human liver surgical wastes, unused transplantable livers, and diseased livers from transplant recipients. The modified procedures for isolation involved collagenase perfusion of a sample of limited size (<50 g) with only one cut surface, perfusion via only one blood vessel, the use of an appropriate amount of collagenase, as well as manual “massaging” of the liver sample during critical stages of the collagenase perfusion. Using this modified procedures, we had close to 100% success rate in the isolation of viable hepatocytes from the human liver samples.
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