A fluorimetric and enzymatic method for the estimation of serum total bile acids
GM Murphy, BH Billing, DN Baron - Journal of clinical Pathology, 1970 - jcp.bmj.com
GM Murphy, BH Billing, DN Baron
Journal of clinical Pathology, 1970•jcp.bmj.comThe enzymatic technique using 3α-hydroxysteroid dehydrogenase for determining bile acids
in blood has been modified by measuring the reduced nicotinamide adenine dinucleotide
fluorimetrically. The increased sensitivity attained enables the concentration of total bile
acids in serum to be estimated using 3 ml for normal subjects and 1 ml for jaundiced
patients. The range of normal values in serum was found to be 0-4· 7 μmol/litre for males
and 1· 0-8· 2 μmol/litre for females.
in blood has been modified by measuring the reduced nicotinamide adenine dinucleotide
fluorimetrically. The increased sensitivity attained enables the concentration of total bile
acids in serum to be estimated using 3 ml for normal subjects and 1 ml for jaundiced
patients. The range of normal values in serum was found to be 0-4· 7 μmol/litre for males
and 1· 0-8· 2 μmol/litre for females.
The enzymatic technique using 3α-hydroxysteroid dehydrogenase for determining bile acids in blood has been modified by measuring the reduced nicotinamide adenine dinucleotide fluorimetrically. The increased sensitivity attained enables the concentration of total bile acids in serum to be estimated using 3 ml for normal subjects and 1 ml for jaundiced patients. The range of normal values in serum was found to be 0-4·7 μmol/litre for males and 1·0-8·2 μmol/litre for females.
jcp.bmj.com
